Materials and methods

Expanded Materials and Methods

Male Wistar rats (200-300 g) were anesthetized by intraperitoneal injection of sodium pentobarbital (90 mg.kg). The hearts were rapidly removed and placed in a cold (4°C) oxygenated Bicarbonate-buffered solution (in mM): NaCl 125; KCl 5; MgSO4 2; KH2PO4 2; NaHCO3 25; glucose 10. They were cannulated through the aorta on a Langendorff column and retrogradely perfused at a constant flow and at 37...

Supplementary materials and methods

Supplementary materials and methods

The human breast cancer cell lines MDA-MB-436 and MDA-MB-231, the murine breast cancer cell line 4T1-luc (4T1 cells stably expressing firefly luciferase) and murine Lewis lung carcinoma cells (LLC) were cultivated according to supplier’s instructions (ATCC). The primary low passaged colon cancer cell lines COGA2 and COGA10 were characterized by Vecsey-Semjen et al. and cultured as previously de...

Supplementary materials and methods

Supplemental Materials and Methods

Recombinant proteins and adenoviral vectors―Recombinant human (rh) VEGF-A165 is provided from R&D Systems (Minneapolis, MN). rhVEGF-C was kind gift from professor Kali Alitalo (Haartman Institute, Helsinki, Finland). For rhVEGF-D N C production the DNA sequences encoding human tissue plasminogen activator (tPA) signal peptide (amino acids 1-21), human VEGF-D mature form (amino acids 93-201) and...